Fig. 2. DZNep induced apoptosis in chondrosarcoma cells but not in chondrocytes. (A) CH2879, SW1353, JJ012, and FS090 chondrosarcomas cell lines as well as CS121030, CS141110, and CS150409 primary chondrosarcomas were treated with DZNep (1 µM) for 14 days. Treatment was renewed at each medium change (days 4, 7, 10) and adherent cells regularly counted. Data are expressed as means + SEM. (B) Chondrosarcomas and chondrocytes were treated with DZNep (1 µM) for 7 days. After treatment, proteins were extracted and PARP cleavage was analyzed by Western blot. Fig.s show a representative image of three independent experiments. (C) Chondrosarcomas were treated as previously. At the end of incubation, caspase 3/7 activity was assayed. Fig.s show a representative image of three independent experiments. Data are expressed as means + SEM (n=3).*: p-value < 0.05; **: p-value < 0.01; ***: p-value < 0.001.